Synergistic pH effect for reversible shuttling aptamer-based biosensors between graphene oxide and target molecules
Loading...
Date
2011-06-07
Authors
Huang, Po-Jung Jimmy
Kempaiah, Ravindra
Liu, Juewen
Journal Title
Journal ISSN
Volume Title
Publisher
Royal Society of Chemistry
Abstract
DNA aptamers are known to desorb from graphene oxide (GO) surface in the presence of target molecules. We demonstrate herein that the binding equilibrium can be shifted by simply tuning the solution pH. At lower pH, the aptamer/GO binding is enhanced while aptamer/target binding is weakened, making this system a regenerable biosensor without covalent conjugation.
Description
Keywords
pH, biosensors, graphene oxide, DNA