Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry
| dc.contributor.author | Huang, Po-Jung Jimmy | |
| dc.contributor.author | Liu, Juewen | |
| dc.date.accessioned | 2017-03-15T15:28:20Z | |
| dc.date.available | 2017-03-15T15:28:20Z | |
| dc.date.issued | 2011-04-26 | |
| dc.description | This is the peer reviewed version of the following article: Huang, P.-J. J., & Liu, J. (2011). Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry. Chemistry - A European Journal, 17(18), 5004–5010, which has been published in final form at https://doi.org/10.1002/chem.201002934. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | en |
| dc.description.abstract | Mercury detection in water has attracted a lot of research interest due to its highly toxic nature and adverse environmental impact. In particular, the recent discovery of specific binding of HgII to thymine-rich (T-rich) DNA resulting in T-HgII-T base pairs has led to the development of a number of sensors with different signaling mechanisms. However, the majority of such sensors were non-immobilized. Immobilization, on the other hand, allows active mercury adsorption, signal amplification, and sensor regeneration. In this work, we immobilized a thymine-rich DNA on a magnetic microparticle (MMP) surface through biotin–streptavidin interactions. In the presence of HgII, the DNA changes from a random coil structure into a hairpin, upon which SYBR Green I binds to emit green fluorescence. Detection was carried out by using flow cytometry where the fluorescence intensity increased ≈9-fold in the presence of mercury and the binding of mercury reached equilibrium in less than 2 min. The sensor showed a unique sample-volume-dependent fluorescence signal change where a higher fluorescence was obtained with a larger sample volume, suggesting that the particles can actively adsorb HgII. Detection limits of 5 nM (1 ppb) and 14 nM (2.8 ppb) were achieved in pure buffer and in mercury-spiked Lake Ontario water samples, respectively. | en |
| dc.description.sponsorship | University of Waterloo || Natural Sciences and Engineering Research Council || | en |
| dc.identifier.uri | http://dx.doi.org/10.1002/chem.201002934 | |
| dc.identifier.uri | http://hdl.handle.net/10012/11487 | |
| dc.language.iso | en | en |
| dc.publisher | Wiley | en |
| dc.subject | DNA | en |
| dc.subject | Mercury | en |
| dc.subject | Flow cytometry | en |
| dc.subject | Immobilization | en |
| dc.subject | Fluorescence | en |
| dc.subject | Magnetic microparticles | en |
| dc.title | Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry | en |
| dc.type | Article | en |
| dcterms.bibliographicCitation | Huang, P.-J. J. and Liu, J. (2011), Immobilization of DNA on Magnetic Microparticles for Mercury Enrichment and Detection with Flow Cytometry. Chem. Eur. J., 17: 5004–5010. doi:10.1002/chem.201002934 | en |
| uws.contributor.affiliation1 | Faculty of Science | en |
| uws.contributor.affiliation2 | Chemistry | en |
| uws.contributor.affiliation2 | Waterloo Institute for Nanotechnology (WIN) | en |
| uws.peerReviewStatus | Reviewed | en |
| uws.scholarLevel | Faculty | en |
| uws.typeOfResource | Text | en |